INVITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF CLOVE BUDS( EUGINEA AROMATICA)

A.Mounika A.Mounika; M.Sushma  M.Sushma; Lahari Sidde Lahari Sidde; Sankara Malathi Sankara Malathi; Konapalli Rajani Konapalli Rajani

doi:10.46956/ijihd.vi.95

A.Mounika Assistant professor, Department of Pharmacology, JNTUA OTPRI, Anantapur, Andhra Pradesh, India.
M.Sushma Assistant professor, Department of Pharmacology, JNTUA OTPRI, Anantapur, Andhra Pradesh, India.
Lahari Sidde Assistant professor, Department of Pharmacology, JNTUA OTPRI, Anantapur, Andhra Pradesh, India.
Sankara Malathi Assistant professor, Department of Pharmacology, JNTUA OTPRI, Anantapur, Andhra Pradesh, India.
Konapalli Rajani Assistant professor, Department of Pharmacology, JNTUA OTPRI, Anantapur, Andhra Pradesh, India.

DOI https://doi.org/10.46956/ijihd.vi.95

Abstract

Bioactive compounds from Eugenia aromaticum were extracted by Soxhlet using DCM ,DCM;WATER,WATER and extracts were examined for its phytocomponents along with Clove oil. These bioactive plant compounds were screened for possible antimicrobial activities against two strains of MDR S. aureus. Antimicrobial activity of extracts using agar diffusion disc variant method. The objective of this study was to evaluate technical variants used in screening methods to determine antibacterial activity of natural products. Thus, a varied range of natural products of plant were tested against two bacterial species,m Staphylococcus aureus ATCC 25923 and klebesella pnemonia ATCC 25922, by two variants of the agar diffusion method (well and disc), two variants of the bio autographic method (direct and indirect) and by microdilution assay. We concluded that the well-variant of the diffusion method was more sensitive than the disc-variant, it was found extract 1 ie,DCM and extract 2 i.e DCM;WATER had shown best results on antimicrobial activity ,phytocvhemical screening test are done for three extractsn invitro evalvation of antimicrobial activity was performed.

Keywords: Agar diffusion, antimicrobial activity, bioautographic methods, minimum inhibitory concentration, minimum bactericidal concentration, natural products

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