International Journal of Health Care and Biological Sciences https://www.saap.org.in/journals/index.php/ijhcbs <p>International Journal of Health Care and Biological Sciences (IJHCBS) is an international online open access, peer-reviewed, quarterly journal Publish Health as well as Life sciences field on the basis of its originality, and serve as a means for scientific information exchange in the international Health Care and Biological science with a strong emphasis on originality and scientific quality. IJHCBS aims to publish quarterly and accepts for publishing original and research articles, review articles, case studies, and reports short communication, meta-analysis.</p> International Journal of Health Care and Biological Sciences en-US International Journal of Health Care and Biological Sciences 2582-7499 Validated RP-HPLC method for the simultaneous estimation of Proguanil and Atovaquone in pharmaceutical dosage form https://www.saap.org.in/journals/index.php/ijhcbs/article/view/549 <p>The aim of the study was to develop and validate a rapid, sensitive and accurate method for simultaneous estimation of proguanil and atovaquone in Pharmaceutical dosage forms by liquid chromatography.The chromatographic separation was achieved on Kromasil C18 (4.6 x 150 mm; 5 µm) at ambient temperature.The separation was achievedby employing a mobile phase consists of Phosphate buffer: acetonitrile (40:60 v/v). The flow rate was 1.0ml/ minute and ultra violet detector at 280 nm. The retention time for proguanil and atovaquone found to be 2.15 min and 2.48 min respectively. The proposed method was validated for selectivity, precision, linearity and accuracy. All the results obtained from various validation parameters were within the acceptable range. The method was found to be linear from concentrations of 20-120 µg/ml for proguanil and 50 - 300µg/ml for atovaquone.</p> Mahendra U Kanchan Sunil Nautiyal Copyright (c) 2023 https://creativecommons.org/licenses/by-nc/4.0 2023-10-25 2023-10-25 1 6 10.46795/ijhcbs.v4i4.549 Case Report on Pulmonary mucormycosis https://www.saap.org.in/journals/index.php/ijhcbs/article/view/550 <p>Pulmonary mucormycosis (PM) is an uncommon fungal infection most often seen in immunocompromised patients. The fungus grows on decaying food, soil, and animal excrement. Patients usually become infected by inhalation of spores. The most common risk factors include diabetes mellitus, hematologic malignancy, and solid organ or stem cell transplant. PM can have a nonspecific appearance at imaging. For example, early imaging may show peribronchial ground-glass opacity. Later, the disease progresses to consolidation, nodules, or masses. Because patients are usually immunocompromised, the differential diagnosis often includes invasive pulmonary aspergillosis (IPA). Various radiologic findings suggestive of PM have been identified to help differentiate it from IPA. For example, the reverse halo sign is more closely associated with PM than with IPA. The reverse halo sign is an area of ground-glass opacity surrounded by a rim of consolidation. In addition, the presence of pleural effusions and more than 10 nodules is more suggestive of PM than it is of IPA. PM can progress rapidly in neutropenic patients. Identification of the hyphae in tissue by using endobronchial or percutaneous sampling can allow differentiation from IPA and help confirm the diagnosis of mucormycosis. Because of the high mortality rate associated with PM, early identification of the disease is critical for an improved likelihood of survival. A multimodality treatment approach with antifungal agents and surgical débridement has been shown to improve outcomes. The authors review the risk factors for PM, describe its imaging appearance and disease process, and describe the treatment of the disease</p> Vamsi V SomaSekhar K Sandeep R Prabhu Kumar K Meghana B Sharmila S Narayana swamy P Copyright (c) 2023 https://creativecommons.org/licenses/by-nc/4.0 2023-11-25 2023-11-25 7 9 10.46795/ijhcbs.v4i4.550